ChemStation PDF Agilent guide to using ChemStation 5973 MSD Guide PDF This CIF guide for using the 5973 MSD, ChemStation, and MassHunter Snagit 10 Starts screen-capture; capture images of errors to paste into Word Data Acquisition Click on the MSD icon to open the acquisition program. The software uses the concept of “tune files”.
I'm using Chemstation for LC 3d Systems Rev B.04.03[16] with unique folder creation turned on. After an impurities run, I have gone through and done all necessary manual integration through the navigation pane and saved it with the data file each time. I'd like to see a batch file that included my manual integration but this doesn't seem possible since I didn't do the manual integration within the batch. Alternately, I'd like to be able to output a summary report similar to the batch report without using a batch. Preferably the summary report would be in Excel format, or in a format that is easy to copy into Excel.
All I need in the report is the Compound name, area, retention time and height, laid out horizontally like it would be in a batch report. Imazing kod aktivacii. Any suggestions? I should note the following: I'm in a research environment, manual integration is definitely acceptable The method is looking for drug impurities and potential leachables from a medical device. The sample injection volume is high so that the imputies peaks show up, the two main compounds in the samples are not of interest. Manual integration is necessary because the chromatograms are often so entirely different among samples that a single set of integration parameters cannot be made to work for all samples. The reason I'd like to know this: this is my first time using this version of chemstation where manual integration changes done in the navigation pane and stored with the data file are completely seperate from manual integration done when reviewing the batch.
I did not know this, I thought that if I did all my manual integration in the navigation pane and saved it to the each data file then when I opened the core batch file it would contain the manual adjustments. This is not the case. Even if I reprocess the data with the manual integration saved to each data file, which re-creates the core.b batch file, the batch will not contain the manual integration. The problem is that I've already done 16 runs over 10 weeks of a 26 week stability study and I don't want to have to go back and open each run as a batch and then re-do the manual integration for all ~6000 samples when I already have the integration done and saved with the data file. But I can't see any way to get anything equivalent to the batch report based on the modified data files.
I'm not able to get sequence summary reporting to do it right, even as a text file (sequence summary reports won't print as Excel files). And I don't even know where to begin with macros. Any help would be greatly appreciated, I'm looking at having to re-do about 3 weeks of work here.
If you make changes in main(acquisition) method for manual integration and save it. After that in Preferences menu go to Signal/Review options and mark in Field 'Method used for review of Sequence data/Single run data' -Current Method. From now on when your method is opened and You open data file, should be integrated as You specified in original method ( main method for analyses on samples).
That is my way of working. I hope so to help. Thank you Danail Y. For providing this response through LinkedIN.
This will be a quick tutorial on how to build a calibration table in Chemstation, with screenshots. The first thing that you want to do is run your standards. Most of the time you want to have 3 levels; low, medium, and high.
You want your compound of interest to have a concentration close to the mid-range level. For example, if my samples will typically have a concentration of 50 ng/ul, then my standards could be 25, 50, and 75 ng/ul. Some people might have five or more levels, others might just have one level.
Next step is integration, you want to be sure that your peaks of interest are all integrated, and you can filter for unwanted peaks (possible blog post about that process). After running your standards, and integrating, the next step would be to select calibration, and new calibration table. I usually leave the settings with the default and hit ok. Now, every single peak that was integrated earlier will show up on your calibration table. Unwanted peaks can be removed by keeping zero as the amount. You can tell the integrated peaks by the pink line beneath the peak and the retention time label above it. At this point, when you click on each line, you can see which peak you are on and give it a name and an amount.
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ChemStation PDF Agilent guide to using ChemStation 5973 MSD Guide PDF This CIF guide for using the 5973 MSD, ChemStation, and MassHunter Snagit 10 Starts screen-capture; capture images of errors to paste into Word Data Acquisition Click on the MSD icon to open the acquisition program. The software uses the concept of “tune files”.
I'm using Chemstation for LC 3d Systems Rev B.04.03[16] with unique folder creation turned on. After an impurities run, I have gone through and done all necessary manual integration through the navigation pane and saved it with the data file each time. I'd like to see a batch file that included my manual integration but this doesn't seem possible since I didn't do the manual integration within the batch. Alternately, I'd like to be able to output a summary report similar to the batch report without using a batch. Preferably the summary report would be in Excel format, or in a format that is easy to copy into Excel.
All I need in the report is the Compound name, area, retention time and height, laid out horizontally like it would be in a batch report. Imazing kod aktivacii. Any suggestions? I should note the following: I'm in a research environment, manual integration is definitely acceptable The method is looking for drug impurities and potential leachables from a medical device. The sample injection volume is high so that the imputies peaks show up, the two main compounds in the samples are not of interest. Manual integration is necessary because the chromatograms are often so entirely different among samples that a single set of integration parameters cannot be made to work for all samples. The reason I'd like to know this: this is my first time using this version of chemstation where manual integration changes done in the navigation pane and stored with the data file are completely seperate from manual integration done when reviewing the batch.
I did not know this, I thought that if I did all my manual integration in the navigation pane and saved it to the each data file then when I opened the core batch file it would contain the manual adjustments. This is not the case. Even if I reprocess the data with the manual integration saved to each data file, which re-creates the core.b batch file, the batch will not contain the manual integration. The problem is that I've already done 16 runs over 10 weeks of a 26 week stability study and I don't want to have to go back and open each run as a batch and then re-do the manual integration for all ~6000 samples when I already have the integration done and saved with the data file. But I can't see any way to get anything equivalent to the batch report based on the modified data files.
I'm not able to get sequence summary reporting to do it right, even as a text file (sequence summary reports won't print as Excel files). And I don't even know where to begin with macros. Any help would be greatly appreciated, I'm looking at having to re-do about 3 weeks of work here.
If you make changes in main(acquisition) method for manual integration and save it. After that in Preferences menu go to Signal/Review options and mark in Field 'Method used for review of Sequence data/Single run data' -Current Method. From now on when your method is opened and You open data file, should be integrated as You specified in original method ( main method for analyses on samples).
That is my way of working. I hope so to help. Thank you Danail Y. For providing this response through LinkedIN.
This will be a quick tutorial on how to build a calibration table in Chemstation, with screenshots. The first thing that you want to do is run your standards. Most of the time you want to have 3 levels; low, medium, and high.
You want your compound of interest to have a concentration close to the mid-range level. For example, if my samples will typically have a concentration of 50 ng/ul, then my standards could be 25, 50, and 75 ng/ul. Some people might have five or more levels, others might just have one level.
Next step is integration, you want to be sure that your peaks of interest are all integrated, and you can filter for unwanted peaks (possible blog post about that process). After running your standards, and integrating, the next step would be to select calibration, and new calibration table. I usually leave the settings with the default and hit ok. Now, every single peak that was integrated earlier will show up on your calibration table. Unwanted peaks can be removed by keeping zero as the amount. You can tell the integrated peaks by the pink line beneath the peak and the retention time label above it. At this point, when you click on each line, you can see which peak you are on and give it a name and an amount.